Fig. 9.

Pretreatment with sanguinarine or CsA does not prevent VP-induced AQP2 plasma membrane accumulation in principal cells of the collecting duct. Kidney slices from adult Sprague-Dawley rats were incubated with sanguinarine (25 μM) or CsA (140 nM) for 40 min. At 15 min before the end of the experiment, VP (100 nM) was added to the kidney slices. Top: in the cortex, AQP2 accumulates on the apical plasma membrane after VP treatment. Pretreatment with CsA or sanguinarine did not affect this AQP2 accumulation on the apical plasma membrane. Cytoplasmic AQP2 staining is visible in control (CT) slices. Bottom: similar results in the inner medulla. Images are representative of 3 independent experiments. Scale bar = 25 μm.