Fig. 2.

Cardiac dosimetry. A: representative serial SPECT/CT images after ¹²⁵I tail vein injection at 9 wk of age (4 wk after AAV9-rNIS injection at 5 × 10¹⁰ vg). B: group data for rats injected with 5 × 10¹⁰ vg of AAV9-rNIS (n = 4) or PBS (n = 1) demonstrated time-dependent decay of cardiac ¹²⁵I accumulation in AAV9-rNIS-injected rats but no cardiac accumulation in rats injected with PBS (control). C: calculation of biological cardiac half-life of ¹²⁵I (see text). D: group data for the 1-h ¹²⁵I uptake obtained using SPECT/CT in AAV9-rNIS-injected rats used for dosimetry and rats from the study groups to confirmed consistent NIS expression and peak ¹²⁵I uptake.