Fig. 2.

Expression of mitochondrial acetylation regulators in cardiac mitochondria. HFD feeding led to a proacetylation phenotype in cardiac mitochondrial proteins. A and B: mitochondrial acetyltransferase Gcn5l1 and deacetylase sirtuin 3 (Sirt3) mRNA expression were significantly increased in cardiac mitochondria from chronic HFD-fed mice. C–E: whereas the GCN5L1 protein level was significantly increased in HFD-fed animals, SIRT3 protein abundance was significantly decreased. Western blots for GCN5L1 and SIRT3 were obtained from the same gel, and therefore the same GDH loading control was used for each blot. G: there was no significant difference in the abundance of the 40-kDa, preimport, cytosolic form of SIRT3 in chow- and HFD-fed mice. H: in contrast, there was a significant decrease in the 28-kDa mitochondrial form of the enzyme in HFD-fed animals. I and J: Gcn5l1 gene expression in H9C2 cells was significantly increased after 4 h of high glucose (25 mM) and palmitate (200 μM) exposure in basal DMEM relative to low glucose (5 mM) exposure, whereas Sirt3 expression was only elevated in response to palmitate. Values are expressed as means ± SE; n = 4 per group. P value significance is as shown in the graphs using two-way Student’s t-test.