Skip to main content
. 2017 May 26;313(2):H283–H292. doi: 10.1152/ajpheart.00813.2016

Fig. 7.

Fig. 7.

Left: primary fibroblasts from the patient with the K475E mutation showed activated cell growth signaling pathways. Fibroblasts from the patient with the K475E mutation and multiple controls were cultured. Western blots were performed on cell lysates with an antibody against phospho-p70S6K (T389), phospho-p70S6K (T421/S424), phospho-S6 (S235/S236), phospho-S6 (S240/S244), phospho-4E-BP1 (T37/T46), phospho-4E-BP1 (S65), phospho-4E-BP1 (T70), phospho-Akt (S473), or phospho-Akt (T308). Blots of each individual total protein (p70S6K, S6, 4E-BP1, and Akt) were also included. A–D, right: densitometric scanning results of Western blots of primary fibroblasts from the patient with the K475E mutation and multiple age- and/or sex-matched controls. Each bar represents the measurement of each phospho-specific antibody normalized with its individual total antibody from 3−4 separate experiments. *P < 0.05 vs. individual WT.