Fig. 8.

ANG II induces H9c2 cell hypertrophy, which can be reversed by rapamycin treatment. A: H9c2 cells overexpressing human PRKAG2 WT or the K475E mutant were treated with vehicle (control), 200 nM ANG II, and 50 nM rapamycin or ANG II in combination with rapamycin for 24 h before being stained for green fluorescent calcein AM for the microscopic assessment of cell area. B: bar graphs showing quantification results (n = 60–80 cells) from four individual experiments. *P < 0.05 vs. WT within the same treatment group; +P < 0.05 vs. vehicle control.