Fig. 9.

Hypertrophy phenotype induced by overexpression of the K475E mutant in H9c2 cells can be corrected by rapamycin treatment. A: H9c2 cells overexpressing human PRKAG2 WT or the K475E mutant were treated with vehicle (control) or 50 nM rapamycin for the indicated periods of time. Cell area was assessed as described in Fig. 8. Each bar represents the measurement of cell area from 3−4 separate experiments (n = 60–80 cells). *P < 0.05 vs. individual control of the same genotype and treatment day group. B: time course effect of rapamycin treatment presented as cell area reduction (in %) based on the data in A.