Table 1.

Summary of gene set enrichment analysis for Lox^−/− aortas compared with Lox^+/+ aortas, regardless of vascular location and separately for the AA and DA

		Lox−/− vs. Lox+/+		Lox−/− vs. Lox+/+ AA		Lox−/− vs. Lox+/+ DA
Gene Subset	Size	NES	P value	NES	P value	NES	P value
ECM regulators	299	2.16	<0.0001*	1.87	<0.0001*	1.85	<0.0001*
Secreted factors	348	1.69	<0.0001*	2.01	<0.0001*	1.06	0.294
ECM glycoproteins	193	1.61	<0.0001*	1.22	0.063	1.49	0.002*
SMC cell cycle	15	1.71	0.012*	1.22	0.202	1.66	0.017*
Collagens	43	1.46	0.041*	1.59	0.011*	1.07	0.332
SMC matrix	36	1.16	0.24	1.25	0.137	0.89	0.701
ECM-affiliated proteins	160	1.11	0.210	1.36	0.017*	−1.35	0.03*
Proteoglycans	36	1.08	0.342	1.02	0.401	0.93	0.555
SMC contractility	102	−0.77	0.936	−0.61	0.996	−0.65	0.993

The name of the gene subset, number of genes in the subset (Size), normalized enrichment score (NES), and nominal P values are shown. The gene subsets are ordered from highest to lowest NES values, based on the lysyl oxidase (Lox)^−/− versus Lox^+/+ results regardless of vascular location. Positive NES indicates upregulation of the gene set, whereas negative NES indicates downregulation of the gene set in Lox^−/− compared with Lox^+/+ mice. Extracellular matrix (ECM) regulators were significantly upregulated at both locations. Secreted factors, collagens, and ECM-affiliated proteins genes were significantly upregulated only in the Lox^−/− ascending aorta (AA) compared with the Lox^+/+ AA. ECM glycoproteins and smooth muscle cell (SMC) cell cycle were significantly upregulated, and ECM-affiliated proteins were significantly downregulated only in the Lox^−/− descending aorta (DA) compared with the Lox^+/+ DA. Leading-edge genes for the significantly enriched subsets are listed in Supplemental Tables S1−S13.

^*P < 0.05.