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. 2017 May 4;313(2):L339–L349. doi: 10.1152/ajplung.00273.2016

Fig. 6.

Fig. 6.

Immunoblot analysis of matrix proteins. A: matrix protein levels were measured by immunoblot analysis of lung tissue homogenates of PN28 mice exposed to saline/RA or LPS/O2 and treated with a single dose of AAV9-miR29b(+) or control virus(−) at PN3. Levels of phospho-SMAD 2/3 and total SMAD 2/3 (B), as well as α-SMA, vimentin, and matrix metalloproteinase (MMP)-9 (C), were quantified by densitometry and were analyzed by two-way ANOVA to identify effects of saline/RA or LPS/O2 exposure and AAV9-miR29b(+) or control virus(−) treatment as indicated on the graph; n = 4–6. Tukey’s post hoc analysis indicated differences between groups: *different than saline/RA(−); #different than LPS/O2(−).