Skip to main content
. 2017 May 18;313(2):L371–L383. doi: 10.1152/ajplung.00532.2016

Fig. 2.

Fig. 2.

PDCD4 levels and apoptosis in peroxisome proliferator-activated receptor-γ (PPARγ)-depleted HPASMCs. HPASMCs were transfected with nontargeting scrambled oligonucleotides or PPARγ (20 nM) dicer-substrate short interfering (si)RNA constructs for the first 6 h of 48- or 72-h incubation periods. A: PPARγ protein expression in HPASMCs transfected with PPARγ siRNA. B: PDCD4 mRNA levels in HPASMCs transfected with PPARγ siRNA. C: PDCD4 protein levels in HPASMCs transfected with PPARγ siRNA. D: caspase-3 activity in HPASMCs transfected with nontargeting scrambled oligonucleotides or siPPARγ. E: cytochrome-c levels were analyzed using an ELISA on HPASMCs transfected with nontargeting scrambled (SCR) oligonucleotides or siPPARγ. A and C: representative immunoblots. Bars represent means ± SE for PPARγ, PDCD4, caspase-3 activity, or cytochrome-c abundance; n = 6–7. **P < 0.01 vs. SCR; ***P < 0.001 vs. scrambled (SCR; ****P < 0.0001 vs. SCR.