Fig. 3.

Pharmacological inhibition of PC reduces ASMC contractility. A: ASMCs were prepared in basal media supplemented with 15 μM Sonic Hedgehog pathway inhibitors vismodegib (Smoothened inhibitor) or HPI-4 (inhibits downstream of Smoothened) and then combined with neutralized collagen and allowed to contract for up to 20 h. B: gel areas were measured with ImageJ software (n = 3 gels per group); data presented as means ± SE. C: contracted collagen gels were fixed in 4% PFA, and paraffin sections were stained with acetylated tubulin (Ac tubulin; red); nuclei were stained with DAPI (blue). Images are at ×1,000 magnification.