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. Author manuscript; available in PMC: 2017 Sep 4.
Published in final edited form as: Methods Enzymol. 2016 Dec 5;587:207–225. doi: 10.1016/bs.mie.2016.10.024

Figure 3. Expression of Atg4 homologues and Atg8 homologues.

Figure 3

A. His-tagged human Atg4A, Atg4B and Atg4C, and GST-tagged Atg4D were expressed in E. Coli, and purified using Ni2+-NTA resins, or glutathione sepharose, respectively. B. Four human Atg8 homologues were fused with the His tag at the N-terminus and GST at the C-terminus, expressed in E. Coli and purified using Ni2+-NTA resins. Recombinant proteins were separated by SDS-PAGE and stained with CBB for quantification by densitometry.