Skip to main content
. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Jul 25;1859(10):2058–2067. doi: 10.1016/j.bbamem.2017.07.014

Figure 2.

Figure 2

Diagram of Ste2p showing positions of Cys mutations and modifications introduced to facilitate disulfide cross-linking and intramolecular interactions. “XX” marks the site of the tandem Factor Xa protease cleavage (IEGRIEGR) engineered into ICL1. The FLAG and HIS epitope tags engineered into the C-terminus are also shown. The two endogenous Cys residues (C59 and C252 – hatched circles) were mutated to Ser to generate Cys-less Ste2p. The sites of Cys mutation engineered into the NT and ECL1 regions for disulfide cross-linking (Y26C, N105C, S108C, V109C, Y111C and T114C) are shown in grey circles. The two known glycosylation sites are shown with “ψ” symbol.