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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Jul 25;1859(10):2058–2067. doi: 10.1016/j.bbamem.2017.07.014

Figure 3.

Figure 3

Comparison of the expression levels and signaling activities of the WT and ICL1-Xa2 receptor used in this study. (A) total membranes prepared from the cells expressing wild-type and ICL1-Xa2 constructs were immunoblotted using anti-FLAG antibody. The bottom panel shows the same immunoblot re-probed using antibody against Pma1p, a constitutively expressed plasma membrane protein used as a loading control. (B) The zone of growth inhibition of strains carrying the indicated receptors was measured in response to various concentrations of α-factor. (C) Signaling activities of the constructs determined by pheromone-induced FUS1-lacZ activity. The grey bars represent the constitutive signaling and the black bars represent the α-factor induced signaling activity. The signaling was normalized to that of the wild-type construct.