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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Jul 25;1859(10):2058–2067. doi: 10.1016/j.bbamem.2017.07.014

Figure 5.

Figure 5

Effect of ligand binding on dimerization. Band intensity of dimer and monomer forms of Ste2p was quantified from Western blots (Figs. 4A & C) using Image Lab (version 4.1). The dimer/monomer ratio of the mutants was normalized to that of the ICL1-Xa2 (Labeled F-H-Xa in figure for Flag-His-Xa2 receptor). Black and grey bars represent the ratio of dimer to monomer in the absence or presence of α-factor, respectively. Statistical significance (p<0.05) in the dimer-monomer ratio is indicated by an asterisk.