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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Jul 25;1859(10):2058–2067. doi: 10.1016/j.bbamem.2017.07.014

Table 1. Biological activities of Cys mutants.

Receptor Growth arrest activities (%)1 Fus1-LacZ Induction (%)2
ICL1-Xa2 100 100
Y26C 60 53
N105C 79 43
Y26C/N105C 40 47
S108C 65 30
Y26C/S108C 59 45
V109C 84 89
Y26C/V109C 34 72
Y111C 90 30
Y26C/Y111C 70 64
T114C 87 57
Y26C/T114C 75 39
1

Relative growth arrest activity compared to that of the ICL1-Xa2 receptor at 1.0 g of α-factor applied to a disk (the halo size of the ICL1-Xa2 receptor was 18 mm). The standard deviation (not shown) of the halo activity for each of the receptors was within 10% (three replicates).

2

Relative maximal FUS1-lacZ activity compared to that of the induced activity of ICL1-Xa2 at 1.0 μM of α-factor. The standard deviation of the FUS1-lacZ activity (not shown) was within ±5% for each of the receptors (three replicates).