. Author manuscript; available in PMC: 2018 Oct 1.

Published in final edited form as: Biochim Biophys Acta. 2017 Jul 25;1859(10):2058–2067. doi: 10.1016/j.bbamem.2017.07.014

Table 2. Dimer to monomer ratio of Cys mutants in non-reducing and reducing conditions.

Receptor	Dimer to Monomer Ratio under Non-reducing Conditions^a	Dimer to Monomer Ratio under Reducing Conditions^b
ICL1-Xa₂	1.0	1.0
Y26C^*	27.9	12.3
N105C	2.2	2.3
Y26C/N105C^*	8.0	5.9
S108C	3.1	2.3
Y26C/S108C^*	8.3	4.4
V109C	3.7	2.2
Y26C/V109C^*	5.7	3.4
Y111C	4.4	3.2
Y26C/Y111C^*	8.2	4.5
T114C	1.9	1.3
Y26C/T114C^*	4.1	2.6

Relative dimer to monomer ratio of the Cys mutants as compared to that of the ICL1-Xa₂ in the absence of β-ME.

Relative dimer to monomer ratio of the Cys mutants as compared to that of the ICL1-Xa₂ in the presence of β-ME.

Significant difference (p<0.05) in dimer to monomer ratio of the receptors in non-reducing and reducing conditions is indicated by “*”.

The dimer to monomer ratio of the mutants of the all the mutants was normalized to that of the ICL1-Xa₂. The standard deviation of the relative dimer to monomer ratio for all receptors was within ±0.6.