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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: Methods. 2017 Jun 1;126:112–129. doi: 10.1016/j.ymeth.2017.05.028

Figure 3. Specific degradation of pre-adenylated linker by combined treatment with Yeast 5′ deadenylase and RecJ.

Figure 3

The presence of a reaction component is indicated by a filled circle, the absence of a component by a empty circle. Y5pDA – Yeast 5′ deadenylase. L – 10 bp ladder. The 100 nt band and selected other bands of the 10 bp ladder is indicated on the right-hand side of the gel. Ligated product is indicated. The effect of Yeast 5′ deadenylase and RecJ treatment on the pre-adenylated linker band is indicated by the box.