Table 10.
Composition of the T4 PNK end-healing reaction
| Component | Volume (µl) | Final |
|---|---|---|
| RNA sample | 3.5 | NA |
| T4 PNK Buffer (10X) | 0.5 | 1X |
| T4 PNK (10 U/µl) | 0.5 | 5 U |
| SUPERase*In | 0.5 | 10 U |
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