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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: Methods. 2017 Jun 1;126:112–129. doi: 10.1016/j.ymeth.2017.05.028

Table 11.

Composition of the linker ligation reaction

Component Volume (µl) Final
50% w/v PEG-8000 3.5 17.5%
10X T4 RNA ligase buffer 0.5 1X
Preadenylated linker (20 µM) 0.5 1 µM
T4 Rnl2(tr) K227Q (200 U/µl) 0.5 100 U