Table 12.
Composition of the reverse transcription reaction
| Component | Volume (µl) | Final |
|---|---|---|
| RNA sample & primer | 12 | NA |
| 5X Protoscript II buffer | 4 | 1X |
| dNTPs | 10 mM ea. | 1 |
| 0.1 M DTT | 1 | 5 mM |
| SUPERase*In (20 U/µl) | 1 | 20 U |
| Protoscript II (200 U/µl) | 1 | 20 U |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.