Figure 4.

Effect of AAAG ODN on TNF-α and IL-6 production in skin tissues and WBCs of burn-injured mice. (A) Burn-injured skin tissues and (B) peripheral white blood cells (WBCs) collected at different time points post burn were used to detect the mRNA expression of TNF-α and IL-6 by qPCR. The WBCs harvested at 2 h post burn were treated with monensin in vitro for 2 h, and then subjected to flow cytometric analysis to detect (C) intracellular expression of TNF-α and IL-6 and (D) the percentage of TNF-α or IL-6 positive neutrophils. Data are represented as mean ± SEM (n = 3 mice/group). *P < 0.05, **P < 0.01, ***P < 0.001.