Figure 6.

The interfering role of AAAG ODN on IRF5. (A) Binding of AAAG ODN with IRF5. The proteins in IRF5 expressing HEK293 cells were pulled down using AAAG ODN or control ODN-coated beads, and analyzed by Western blot with IRF5 monoclonal antibodies. (B) Inhibition of AAAG ODN on nuclear translocation of IRF5. The THP-1 cells were incubated with CpG 2006 plus AAAG ODN or control ODN for 24 h, then harvested to separate cytoplasmic and nuclear fractions. The proteins in each fraction were recognized by IRF5 antibodies by Western blot. Nu IRF5: IRF5 in nucleus; Cyto IRF5: IRF5 in cytoplasm. The data are from three independent experiments and are represented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.