Figure 1.

Expression of IL-17A in microglial cells. After transfection with IL-17A siRNA for 24 h, microglial cells were placed in an oxygen deprived incubator at 37°C for 2 h, and then moved to normal conditions to terminate the OGD and start reperfusion for 24 h. IL-17A expression as measured by Western blot analysis in EOC 2 cells (A), EOC 2 culture supernatants (B), and mouse primary microglia culture supernatants (C). Protein concentrations of IL-17A in EOC 2 (D) and mouse primary microglia (E) culture supernatants were determined by ELISA. Experiments were carried out at least in triplicate and the results were expressed as the mean values, *P < 0.05 vs. the control. NC, negative control siRNA.