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. 2017 Aug 31;10:271. doi: 10.3389/fnmol.2017.00271

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Copyright © 2017 Zhang, Yang, Mo, Lin and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of IL-17A regulatory proteins. (A) Typical examples of some differentially expressed protein spots with at least two-fold changes in EOC 2 cells, EOC 2 cells in an OGD model and EOC 2 cells transfected with IL-17A siRNA in OGD model by 2-DE. (B) EOC 2 cells were transfected with IL-17A siRNA for 24 h, then started the OGD for 2 h and reperfusion for 24 h, and the expression of cleaved-caspase-3, HMGB1 and osteopontin was measured by Western blot analysis. *P < 0.05 vs. the control.