Figure 8.

Expression of HMGB1 and IL-17A, and caspase 3 activity in microglial cells. After transfection with IL-17A or HMGB1 siRNA for 24 h, microglial cells were started in the OGD condition for 2 h and reperfusion for 24 h. HMGB1 and/or IL-17A expression as measured by Western blot analysis in EOC 2 culture supernatants (A), mouse primary microglia culture supernatants (B), and EOC 2 cells (C). Protein concentrations of HMGB1 in EOC 2 (D) and mouse primary microglia (E) culture supernatants were determined by ELISA. (F) The activity of caspase-3 was monitored by the fluorometric method. *P < 0.05 vs. the control.