Table 1.
Protein name | Accessionb | MW | pI | Scorec | Coverage (%) | No. peptides | Pep matchd |
---|---|---|---|---|---|---|---|
DOWN-REGULATED PROTEINS | |||||||
Dihydropyrimidinase-related protein 2 | DPYL2_MOUSE | 62,638 | 5.95 | 661 | 43 | 19 | 107 |
Heterogeneous nuclear ribonucleoprotein K | B2M1R6_MOUSE | 48,760 | 5.38 | 466 | 32 | 15 | 77 |
RuvB-like 2 | RUVB2_MOUSE | 51,252 | 5.49 | 671 | 47 | 22 | 110 |
NSFL1 cofactor p47 | NSF1C_MOUSE | 40,655 | 5.04 | 626 | 54 | 17 | 140 |
Serine/threonine-protein phosphatase | Q8BN07_MOUSE | 33,136 | 5.37 | 476 | 58 | 15 | 89 |
Thioredoxin-like protein 1 | TXNL1_MOUSE | 32,616 | 4.84 | 622 | 47 | 12 | 150 |
Eukaryotic translation initiation factor 6 | IF6_MOUSE | 27,007 | 4.63 | 482 | 52 | 8 | 118 |
Elongin-B | ELOB_MOUSE | 13,219 | 4.87 | 225 | 77 | 8 | 90 |
Calponin | A0A0G2JDV8_MOUSE | 31,546 | 5.79 | 216 | 37 | 11 | 64 |
Twinfilin-1 | TWF1_MOUSE | 40,283 | 6.21 | 292 | 26 | 9 | 91 |
UP-REGULATED PROTEINS | |||||||
Vimentin | VIME_MOUSE | 53,712 | 5.06 | 230 | 39 | 13 | 54 |
Actin, cytoplasmic 2 | ACTG_MOUSE | 42,108 | 5.31 | 454 | 41 | 11 | 123 |
Ubiquitin thioesterase OTUB1 | OTUB1_MOUSE | 31,478 | 4.85 | 567 | 65 | 14 | 138 |
Phosphoserine phosphatase | SERB_MOUSE | 25,308 | 5.81 | 486 | 58 | 12 | 95 |
Caspase-3 | CASP3_RAT | 31,927 | 5.92 | 280 | 30 | 7 | 105 |
High mobility group protein B1 | HMGB1_RAT | 25,049 | 5.62 | 205 | 29 | 6 | 84 |
Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform | PP2AB_MOUSE | 36,123 | 5.21 | 471 | 51 | 14 | 77 |
BAG family molecular chaperone regulator 2 | BAG2_MOUSE | 23,630 | 6.01 | 292 | 33 | 6 | 68 |
Annexin | B0V2N8_MOUSE | 19,698 | 5.68 | 340 | 43 | 5 | 98 |
Neudesin | NENF_MOUSE | 18,893 | 5.13 | 271 | 54 | 5 | 67 |
Microglial cells were placed in an oxygen deprived (95% N2/3% CO2/2% O2) incubator at 37 °C for 2 h, and then returned to normoxic conditions with regular medium to terminate the OGD and start reperfusion for 48 h and subjected to 2D gel analyses.
Accession numbers of proteins were derived from Uniprot database.
Probability scores are based on the MS/MS analysis.
Number of peptides matched.