Table 1.

Differentially expressed proteins in microglial cells in the model of OGD^a.

Protein name	Accessionb	MW	pI	Scorec	Coverage (%)	No. peptides	Pep matchd
DOWN-REGULATED PROTEINS
Dihydropyrimidinase-related protein 2	DPYL2_MOUSE	62,638	5.95	661	43	19	107
Heterogeneous nuclear ribonucleoprotein K	B2M1R6_MOUSE	48,760	5.38	466	32	15	77
RuvB-like 2	RUVB2_MOUSE	51,252	5.49	671	47	22	110
NSFL1 cofactor p47	NSF1C_MOUSE	40,655	5.04	626	54	17	140
Serine/threonine-protein phosphatase	Q8BN07_MOUSE	33,136	5.37	476	58	15	89
Thioredoxin-like protein 1	TXNL1_MOUSE	32,616	4.84	622	47	12	150
Eukaryotic translation initiation factor 6	IF6_MOUSE	27,007	4.63	482	52	8	118
Elongin-B	ELOB_MOUSE	13,219	4.87	225	77	8	90
Calponin	A0A0G2JDV8_MOUSE	31,546	5.79	216	37	11	64
Twinfilin-1	TWF1_MOUSE	40,283	6.21	292	26	9	91
UP-REGULATED PROTEINS
Vimentin	VIME_MOUSE	53,712	5.06	230	39	13	54
Actin, cytoplasmic 2	ACTG_MOUSE	42,108	5.31	454	41	11	123
Ubiquitin thioesterase OTUB1	OTUB1_MOUSE	31,478	4.85	567	65	14	138
Phosphoserine phosphatase	SERB_MOUSE	25,308	5.81	486	58	12	95
Caspase-3	CASP3_RAT	31,927	5.92	280	30	7	105
High mobility group protein B1	HMGB1_RAT	25,049	5.62	205	29	6	84
Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform	PP2AB_MOUSE	36,123	5.21	471	51	14	77
BAG family molecular chaperone regulator 2	BAG2_MOUSE	23,630	6.01	292	33	6	68
Annexin	B0V2N8_MOUSE	19,698	5.68	340	43	5	98
Neudesin	NENF_MOUSE	18,893	5.13	271	54	5	67

^aMicroglial cells were placed in an oxygen deprived (95% N₂/3% CO₂/2% O₂) incubator at 37 °C for 2 h, and then returned to normoxic conditions with regular medium to terminate the OGD and start reperfusion for 48 h and subjected to 2D gel analyses.

^bAccession numbers of proteins were derived from Uniprot database.

^cProbability scores are based on the MS/MS analysis.

^dNumber of peptides matched.