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. 2017 Sep 4;7:10310. doi: 10.1038/s41598-017-10677-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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T cell proliferation assay. Cultivating CFSE-stained PBMCs in the presence of ES antigens revealed TcES to be capable of reducing PHA-induced lymphocyte proliferation (A) at 150 µg/mL and this was detectable in both CD4+ (B) and CD8+ (C) T cells. AcES showed similar effects in lymphocytes and CD4+ cells, while the anti-proliferative capability was lower with loss of statistical significance in CD8+ T cells. P-values were calculated according to one-way ANOVA followed by the Tukey’s honest significant difference post-hoc test. Error bars represent standard error of mean (SEM). FSC = forward scatter, SSC = side scatter. Experiments were repeated six times at two different time points.