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. 2017 Sep 4;7:10310. doi: 10.1038/s41598-017-10677-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DC maturation assay. Pulsing of moDCs with ES products before LPS stimulation revealed both AcES and TcES to be capable of preventing the upregulation of MHC-II (A) and CD80 (B). For MHC-II, AcES was exceeding the effect of TcES by causing moDCs to downregulate MHC-II expression compared to medium control. Although ES preparations caused downregulation of CD80 compared to medium control this effect was statistically significant only for AcES without subsequent LPS stimulation. P-values were calculated according to paired t-test (for MHC-II) and Wilcoxon signed rank test (for CD80). Error bars show standard error of mean (SEM). Experiments were repeated six times at two different time points.