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. 2017 Sep 4;8:412. doi: 10.1038/s41467-017-00432-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Genome-wide expression analysis of the eye reveals dynamic seasonal changes in phototransduction. a Clustered organization of seven upregulated and two downregulated opsin genes following a long day/warm temperature (LW) stimulus. Data were normalized over the complete data set. The color scale represents the normalized signal intensity. b Seasonal changes in the expression of genes encoding photopigments and their downstream phototransduction pathways. Gene symbols in red and blue indicate up- and downregulated genes, respectively. Adapted from the KEGG (Kyoto Encyclopedia of Genes and Genomes) phototransduction pathway. c In situ hybridization analysis confirmed LW induction of LWS opsin in the photoreceptor layer (GC ganglion cell layer, IN inner nuclear layer, ON outer nuclear layer, PE pigment epithelium, PR photoreceptor layer). Representative images from the central area of the retina (also see Supplementary Fig. 6). White arrow indicates the hybridization signal. Scale bar: 50 µm