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. 2017 Sep 4;7:10393. doi: 10.1038/s41598-017-10927-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nutlin-3a cannot reverse high glucose-induced collagen III and fibronectin accumulation in vitro. (A) Representative western blot images and (B) summarized data showing that the elevation expression of p53 in Nutlin-3a-treated HBZY-1 cells n = 4. ^# P < 0.05 vs. HG + Vehl. (C) Representative western blot images and (D) summarized data showing that Nutlin-3a treatment has no benefit on high glucose-induced collagen III and fibronectin accumulation in cultured HBZY-1 cells n = 7. ^* P < 0.05 vs. Ctrl; ^& P > 0.05 vs. HG + Vehl.