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. 2017 Sep 4;7:10393. doi: 10.1038/s41598-017-10927-5

Figure 5.

Figure 5

Genetic deletion of MDM2 attenuates aberrant Notch1 signaling in high glucose-treated GMCs. (A) Representative western blot images and (B) summarized data showing the protein level of NICD1 and Hes1 in HBZY-1 cells exposed to high glucose for indicated times n ≥ 5. * P < 0.05 vs. Ctrl. (C) Representative western blot images and (D) summarized data showing that the genetic deletion of MDM2 by siRNA effectively suppresses the protein level of NICD1 and Hes1 n = 5. * P < 0.05 vs. Ctrl + Scra; # P < 0.05 vs. HG + Scra. (E) Representative western blot images and (F) summarized data showing the protein level of Hey1 in HBZY-1 cells exposed to high glucose for indicated times n = 6. * P < 0.05 vs. Ctrl. (G) Representative western blot images and (H) summarized data showing that the genetic deletion of MDM2 by siRNA effectively suppresses the protein level of Hey1 n = 3. * P < 0.05 vs. Ctrl + Scra; # P < 0.05 vs. HG + Scra.