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Identification of homolog-specific telomere signals. Metaphase spread after hybridization with telomeric PNA (top) followed by a second hybridization step with polymorphic subtelomeric probes, f7501 and ICRF10 (bottom). Chromosomes are counterstained with DAPI. In this particular donor, the second step allows distinction between homologs for chromosome pairs 7, 8, 9 and 11. Homologs for chromosome pair 1 can be distinguished by the size of their heterochromatin.
