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. 2017 Sep 4;7:10315. doi: 10.1038/s41598-017-10529-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Determination of HSCs and activated HSCs in the livers of male and female xmrk+ and Myc+ fish following oncogene activation. 10 fish were analyzed in each group and the experiment was repeated once for reproducibility. (A) IF co-staining of GFAP (red) and a-SMA (green) in liver sections. White boxes indicate the enlarged area as shown on the right. (B) Quantification of total HSC density in liver sections. (C) Quantification of activated ratio of HSCs in liver sections. *P < 0.05. Scale bars: 20 μm.