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. 2017 Sep 4;7:10369. doi: 10.1038/s41598-017-09948-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Viral factory pattern in PAM. Cells were infected with NHV/P68 and Armenia/07 strains (MOI = 1), fixed at 16hpi and incubated with phalloidin-TRITC, anti-p72 antibody and DAPI to respectively stain actin filaments, viral particles and cellular and viral DNA. Z-slides images were taken by CLSM and represented as a maximum of z-projection. Arrowheads show the viral factories visualized by anti-p72 MAb and with DAPI to stain the viral DNA. Images are representative of two independent experiments.