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. 2017 Sep 4;7:10313. doi: 10.1038/s41598-017-10875-0

Figure 4.

Figure 4

Effect of different epitopes of p17 on its toxicity. (a) Amino acid sequence of p17 and of peptides homologous to different residues (blue). In red the epitopes recognised by the different antibodies. (b) Effect of different p17 peptides on pharyngeal pumping. Nematodes (100 worms/100 µL) were incubated for 2 h with 4 nM peptides. Control worms received 10 mM PB (pH 7.4) (vehicle). Mean ± SE, **P < 0.01 vs. Vehicle, one-way ANOVA and Bonferroni post hoc test. (c) Dose-response effect of 0.01–8 nM p1730–55 peptide. Control worms received vehicle alone (vehicle, dotted line). (d) MBS-12 antibody at 6 ng/µL in 10 mM PB (pH 7.4) was co-incubated for 30 min with 4 nM p17 or myr-p17 before administering to the worms. MBS-12 (6 ng/µL) and 10 mM PB (pH 7.4) alone were used as positive and negative controls, respectively (vehicle). Mean ± SE (n = 20 worms/group). **Pp < 0.01 vs. vehicle and °°P < 0.01 vs. the corresponding p17 or myr-p17, two-way ANOVA and Bonferroni post hoc test.