Fig. 5.

Deletion of MCS-R2 in CD34+ cells from HbE β-thalassemia patients using Cr2 + Cr12. a Frequency of different types of mutations generated. b α/β-globin mRNA ratios of individual clones of erythroid cells which are non-deleted normal control (n = 6), non-deleted HbE β-thalassemia (n = 13), HbE β-thalassemia heterozygous for the deletion of MCS-R2 (n = 20), and HbE β-thalassemia homozygous for the deletion of MCS-R2 (n = 20) analyzed by qPCR; median (horizontal bar) and 95% confidence interval (error bar) are shown and P-values were calculated using Mann–Whitney U-test. c Data for α/(β + γ) globin mRNA ratios for individual clones of HbE β-thalassemia erythroid cells grouped according to genotype and normalized to median of non-deleted HbE β-thalassemia clones; median (horizontal bar) and 95% confidence interval (error bar) are shown and P-values were calculated using Mann–Whitney U-test