Table 1.
Sequence of events and volumes of BGE, sample and sample carrier solution used by the SI-CE system.
| Operational step | Time | HV | Valves | Pump flowrate | Consumption | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Time (s) | HV supply | Selector valve position | Switching valve position | 3-way solenoid to | 2-way solenoid position | BGE (µL.min−1) | Sample + carrier (µL.min−1) | BGE (µL) | Sample (µL) | Carrier (µL) | ||
| 1 | Separation & Sampling | 452 | On | 2 | 1 | Waste | Open | 50 | 8 | 376.7 | 60.25 | 0 |
| 2 | Capillary flushing, sample to H-filter | 115 | Off | 1 | 1 | Waste | Closed | 250 | 90 | 479.2 | 0 | 172.5 |
| 3 | Equilibrate | 2 | Off | 1 | 1 | Waste | Open | 0 | 0 | 0 | 0 | 0 |
| 4 | Extraction (H-filter) | 60 | Off | 1 | 1 | Waste | Open | 50 | 8 | 50 | 0 | 8 |
| 5 | Fill T-interface with sample | 78 | Off | 1 | 2 | Interface | Open | 0 | 180 | 0 | 0 | 234 |
| 6 | Hydrodynamic injection | 1 | Off | 1 | 2 | Interface | Closed | 0 | 10 | 0 | 0 | 0.2 |
| 7 | Fill T-interface with BGE | 12 | Off | 1 | 1 | Waste | Open | 250 | 8 | 50 | 0 | 1.6 |
| TOTAL | 720 | 955.9 | 60.25 | 416.3 | ||||||||