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. 2017 Sep 4;8:423. doi: 10.1038/s41467-017-00448-0

Table 1.

Composition of the culture medium at each stage

Stage Expansion Diff1 Diff2 Diff3 Diff4 Diff5
Day D1–D5 D5–D9 D9–D12 D12–D16 D16–D20 D20–D23
Density 1E5/ml 1E5/ml Dilute into 4X 1E5/ml 1E6/ml 5E6/ml
Medium Stemspan IMDM IMDM IMDM IMDM IMDM
rhFLT3 Plasma Plasma Plasma Plasma Plasma
rhSCF AB serum AB serum AB serum AB serum AB serum
rhIL-3 Heparin Heparin Heparin Heparin Heparin
rhIL-6 Insulin Insulin Insulin Insulin Insulin
Dex Holo-transferrin Holo-transferrin Holo-transferrin Holo-transferrin Holo-transferrin
SCF SCF SCF Epo
IL-3 IL-3 Epo
Epo Epo