Table 2.
Indirect quantification of the number of neutrophil-associated bacteria determined as the mean fluorescence intensity emitted by the FITC-labeled bacteria after phagocytosis. The numbers in bold highlights the antibodies that mediated higher bacterial attachment and phagocytosis by neutrophils. Different letters (a, b or c) indicates statistically different results (p < 0.05) within the column.
| Antiserum | Mean of fluorescence intensity of neutrophils after 15 minutes of phagocytosis | |||||
|---|---|---|---|---|---|---|
| SV1 (N°4) | SV4 (SW124) | SV5 (Nagasaki) | SV12 (H425) | SV14 (84-22113) | SV15 (84-15995) | |
| TbpB-I | 1,779 ± 45d | 475 ± 20a | 5,067 ± 352b | 1,194 ± 37b | 868 ± 68b | 541 ± 19ab | 
| TbpB-M | 2,101 ± 77 c | 519 ± 2 6 a | 6,798 ± 128 b | 1,297 ± 27 b | 976 ± 40 ab | 548 ± 28ab | 
| TbpB-Nm | 1,353 ± 29b | 467 ± 25a | 5,005 ± 343b | 1,035 ± 101ab | 809 ± 76ab | 513 ± 41ab | 
| TbpB-C | 1,461 ± 35b | 514 ± 44a | 6,554 ± 204b | 1,066 ± 21ab | 942 ± 32b | 575 ± 18 b | 
| PBS-IMS | 380 ± 10a | 419 ± 12a | 2,655 ± 359a | 857 ± 82a | 668 ± 42a | 425 ± 23a |