Table 3.

Activation of classical pathway of complement system by sera against TbpBs produced in mice. Antibodies from mice immunized with wild-type TbpB, TbpB-M, TbpB-C and TbpB-Nm adjuvanted with Montanide IMS 2215 VG PR, Montanide Gel 01 or Freund’s adjuvant were tested against 6 virulent serovars of H. parasuis. The capacity of these sera to activate the complement system is represented as percentage of activation.

Adjuvant	Reference serovar	Antigens classical pathway activation of complement system (%)
		TbpB-I	TbpB-M	TbpB-C	TbpB-Nm
Montanide IMS 2215 VG PR	1	0	0	0	0
	4	0	0	0	0
	5	0	0	0	0
	12	0	0	0	0
	14	0	0	0	0
	15	0	0	0	0
Montanide Gel 01 PR	1	20	100	100	100
	4	80	100	100	100
	5	50	100	100	100
	12	0	100	100	100
	14	0	100	100	100
	15	0	100	100	100
Freund’s adjuvant	1	50	100	100	100
	4	80	100	100	100
	5	50	100	100	100
	12	0	100	100	100
	14	0	100	100	100
	15	0	100	100	100