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. 2017 Sep 4;7:10332. doi: 10.1038/s41598-017-10611-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RAGE blockade reduced Nestin-positive cells expressing MAP-2 1 week after SCI. Figures with the blue fluorescence dye stand for the nucleus. Figures with the green fluorescence stand for nestin-positive cells. Figures with red fluorescence stand for MAP-2-positive cells. Figures merged with DAPI/NESTIN/RAGE showed double-positive cells. In the sham group, nestin expression was seldom found, but after SCI, nestin expression was significantly enhanced and co-expressed with MAP-2. In the anti-RAGE group, we observed the reduced nestin-positive cells were expressed with less MAP-2. Higher magnification shows the area of a white square with magnification of *1000. Scar bar was 50 µm. The white arrow shows positive expression cells. (b) Relative immunofluorescence intensity of SOX-2 expression. (c) Relative immunofluorescence intensity of RAGE expression. (d) Percentage of SOX-2/RAGE double-positive cell (%).