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. 2017 Sep 4;7:10306. doi: 10.1038/s41598-017-10855-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of LPS on expression of ARIP2 in Raw264.7 cells. (A) ARIP2 mRNA expression was examined by RT-PCR in Raw264.7 cells treated with LPS for 12 h. Lane 1, Control; Lane 2, LPS 200 ng/ml; Lane 3, LPS 500 ng/ml. The graph represented the relative levels of ARIP2 mRNA in three separate experiments, and the mRNA levels of control group were adjusted to 100%. *P < 0.05, **P < 0.01, compared with control. (B) Expression of mature ARIP2 protein in Raw264.7 cells were detected by immunocytochemical staining with normal rabbit IgG as control staining (a) and with anti-ARIP2 antibody 12 h after untreated (b) or treated with LPS 500 ng/ml (c). Arrows represented ARIP2 positive cells (200×). (C) Levels of ARIP2 protein expression were examined by Western blotting in Raw264.7 cells treated with LPS for 12 h. Lane 1, Control; Lane 2, LPS 200 ng/ml; Lane 3, LPS 500 ng/ml. The graph represented the relative levels of ARIP2 protein in three separate experiments, and the protein levels of control group were adjusted to 100%. **P < 0.01, compared with control. Full-length blots/gels are presented in Supplementary Figure 2.