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. 2017 Sep 4;7:10334. doi: 10.1038/s41598-017-10509-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of IL-17 receptors in DRG neurones in WT mice. (a) Representative agarose gel images of PCR products of IL-17 receptor subtypes in dorsal root ganglion (DRG) neurones of naïve WT mice. In WT mice a strong expression of IL-17RA, IL-17RC and IL-17RD and a weak expression of IL-17RB and IL-17RE is detectable. (b) Typical labelling of IL-17 receptors in DRG sections from naïve WT mice with anti-IL-17RA, -IL-17RB, -IL-17RC, -IL-17RD and anti-IL17RE antibodies. Arrows indicate representative cells considered positively labelled. Insets display labelling of sections at higher magnification and show that all the receptor subtypes were localised in subsets of neurones of different sizes (asterisks indicate all cells considered positively labelled) whereas IL-17RA and IL-17RC were also localised in satellite cells (exemplary cells marked by red triangles). Control staining only with rabbit-anti-goat biotinylated antibody (RAG) and goat-anti-rabbit biotinylated antibody (GAR). Magnification 200x (bars 15 µm), insets 400x. Values are mean ± SEM. IL-17R IL-17 receptor, H ₂0, water control, bp base pairs.