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. 2017 Sep 4;7:10334. doi: 10.1038/s41598-017-10509-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PCR and RT-PCR of IL-17 cytokine family members in knee joint tissues at day 2 of AIA. (a) Representative PCR-ScreenTape (Agilent 2200 TapeStation) of IL-17 cytokine family members in tissues of WT and IL-17AKO mice. PCR analysis does not give information about expression quantity. Upper (magenta) and lower (green) markers are used as internal references to determine the molecular weight size of the sample. DNA ladder (25–1500 bp) on the left. GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) serves as a housekeeping control gene. (b) Quantitative reverse transcriptase (RT)-PCR in tissues of sympathectomised IL-17AKO mice and IL-17AKO AIA control mice (n = 5 per group). mRNA level after sympathectomy are given in relation to non-sympathectomised control mice. Sympathectomy significantly (p = 0.012) decreases IL-17D-mRNA. Values are mean ± SEM. *p < 0.05 bp, base pairs; NC, no amplification control.