Figure 4.

Distribution of the Primary Cells Injected

Representative images of the primary cells in the subretinal space. (A) Cross retinal section showing Venus-positive cells near to the photoreceptor layer at the site of injection. (B–D) Cross sectional retinal areas injected with primary cells transfected with Venus (scale bar, 100 μm). (E–H) Flat mount retinas injected with rat RPE cells transfected with Venus (green) and labeled with CellBrite (red) and DAPI (blue) from ONL (E) to RPE (F–H). (I–L) The highlighted with rectangles were examined at higher magnification with confocal super-resolution imaging. CellBrite labeled the plasmatic membrane and DAPI labeled the nuclei. Scale bar, 50 μm. CB, CellBrite; RPE, retinal pigment epithelium; ONL, outer nuclear layer.