Figure 8.

Effect of PEDF-Transfected Rat RPE and IPE Cells on Neovascularization in a Rat Model of Choroidal Neovascularization

Photographs and measurement of CNV area of laser-induced CNV 10 days after treatment with an injection of 5 μL of saline, transplantation with 20,000 PEDF-transfected RPE cells, and 10,000 PEDF-transfected IPE cells. (A) Photographs of lesions. Each row represents 3 different CNV lesions from 3 different animals. Vessels were stained with Caveolin-1 (white). (B and C) Measurement of CNV area. The CNV area in rats transplanted with PEDF-transfected cells is approximately 50% less than that of the saline control (p < 0.05) (B). CNV area of control groups (C). There was no statistical difference among control groups. Area of CNV was calculated using Fiji software, and data were presented as a percentage of mean ± SEM (n = 3) versus the saline group. Scale bar, 50 μm. *p < 0.05.