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. 2017 Sep 4;7:10309. doi: 10.1038/s41598-017-10765-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression pattern and subcellular localisation of GME in Arabidopsis. (A–J) Histochemical GUS activity in seedlings (A), inflorescences (B), flowers (C), pollen grains (D) and pollen tubes (E) from T1 transgenic Arabidopsis expressing the GUS reporter gene under the control of the GME promoter (P _GME::GUS) with GUS activity in wild type (I,J) as a control. (K) and (L) RT-PCR analysis (K) and real-time PCR analysis (L) of the GME expression level in Arabidopsis roots (R), stems (S), stem leaves (SL), rosette leaves (RL) and flowers (F). ACTIN2 was used as the normalisation or internal control. (M) Subcellular localisation of GME in epidermal cells from N. benthamiana leaves. GME-fused GFP driven by the 35S promoter (35S::GME-GFP) and 35S::GFP were expressed in N. benthamiana leaves, respectively. GFP fluorescence was detected 50 h after infiltration. Infiltration with buffer was used as a negative control. BF, bright field. (N) Arabidopsis protoplasts transformed without (Control) or with 35S::GME-GFP. Red fluorescence indicates chloroplasts.