Fig. 1. Glucose-stimulated insulin secretion (GSIS) and viability of MIN6 (mouse insulinoma) cells treated with the free fatty acid (FFA) mixtures corresponding to the different profiles. MIN6 cells were treated for 24 (A, C) or 48 hours (B, D) with the FFA mixtures corresponding to the profiles of adolescents of normal weight (NW), obese adolescents without metabolic syndrome (MetS) (ONMS) or obese adolescents with MetS (OWMS). (A, B) Control cells were grown without adding fatty acids. The percentage change in GSIS is shown. (C, D) Cell viability was determined using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, with the results normalized to the control, and expressed as a viability percentage. ^aIndicates P<0.05, ^bP<0.01, ^cP<0.001 using analysis of variance followed by Tukey post hoc test for multiple comparisons. The cells were treated with methanol as negative control for viability (n=3 independent experiments).