Fig. 2. Mitochondrial function, lipid peroxidation, and antioxidant capacity of MIN6 (mouse insulinoma) cells treated with free fatty acids (FFAs). MIN6 cells were treated for 24 (A, C, E, G) or 48 (B, D, F, H) hours with the FFA mixtures corresponding to the profiles of adolescents with normal weight (NW), obese adolescents without metabolic syndrome (MetS) (ONMS) or obese adolescents with MetS (OWMS). Control cells were grown without adding fatty acids. (A, B) The potential of the internal mitochondrial membrane was quantified as the mean intensity of fluorescence (MIF) emitted by MitoTracker using flow cytometry analysis. (C, D) The adenosine triphosphate/adenosine diphosphate (ATP/ADP) ratio was determined using bioluminescence. (E, F) The production of lipid peroxidation products was quantified using the thiobarbituric acid reactive substance assay and expressed as the concentration of malondialdehyde (MDA) normalized with the total mg of protein. (G, H) The capacity to trap peroxynitrite is expressed relative to the antioxidant activity of ascorbic acid (AA) with normalization to the total protein content of each group. AU, arbitrary unit. aIndicates P<0.05, bP<0.001 using analysis of variance followed by Tukey test of multiple comparisons.
