Fig. 3. Comparison of viral growth kinetics and viral pathogenicity of rL, rL-RVG, and rL-RVGTM. (A) Ten-day-old embryonated eggs were inoculated with rL, rL-RVG, or rL-RVGTM (0.1 mL of 100 EID₅₀), and the allantoic fluid from each group was harvested at different times (12, 24, 36, 48, 60, 72, 84, 96, or 108 h post-infection). The fluid from five eggs per time was pooled for TCID₅₀ determination in BHK cells. The data shown are means of the results from five experiments, and the error bars indicate SD. (B) Pathogenicity of the recombinant viruses was determined in chicken embryos and chicks by determining the mean death time (MDT) in embryonated eggs, the intracerebral pathogenicity index (ICPI) in 1-day-old chickens, and the intravenous pathogenicity index (IVPI) in 6-week-old chickens. (C) Weight changes of mice inoculated with rL-RVG or rL-RVGTM. Two groups of 12 mice were inoculated intramuscularly (i.m.) in the thigh muscle with 5 × 10⁷ EID₅₀ rL-RVG or rL-RVGTM and were observed and weighed daily for 14 days. All mice survived and body weight changes of each group are shown as ratios of the body weight at day 0 (set as 100). RVG, rabies virus G protein; RVGTM, chimeric rabies virus G protein.